3UJ0
Crystal structure of the inositol 1,4,5-trisphosphate receptor with ligand bound form.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210r |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 189.166, 78.720, 134.107 |
Unit cell angles | 90.00, 124.49, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.600 |
R-factor | 0.26 |
Rwork | 0.258 |
R-free | 0.30780 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.116 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.600 |
Number of reflections | 71417 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | Na Citrate, PEG-6000, pH 8.4, vapor diffusion, hanging drop, temperature 293K |