3UH4
TANKYRASE-1 complexed with NVP-XAV939
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-08-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.915, 45.239, 88.135 |
| Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
| Resolution | 44.070 - 2.000 |
| R-factor | 0.1829 |
| Rwork | 0.180 |
| R-free | 0.23380 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BUSTER (2.11.1) |
| Refinement software | BUSTER (2.11.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.285 |
| Number of reflections | 32886 | |
| <I/σ(I)> | 13.62 | 4.02 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 3.3 | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 298 | 15% PEG3350, 290 mM Ammonium Sulfate, 100mM Bis-Tris pH5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
