3UEE
Crystal structure of human Survivin K62A mutant bound to N-terminal histone H3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-06 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 113.888, 70.872, 82.037 |
Unit cell angles | 90.00, 129.52, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.610 |
R-factor | 0.1875 |
Rwork | 0.185 |
R-free | 0.23679 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uec |
RMSD bond length | 0.017 |
RMSD bond angle | 1.790 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.055 | 0.430 |
Number of reflections | 15257 | |
<I/σ(I)> | 38.3 | 2.8 |
Completeness [%] | 99.1 | 93.6 |
Redundancy | 4.1 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 12% PEG 3350, 0.12 M succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |