3UA8
Crystal Structure Analysis of a 6-Amino Quinazolinedione Sulfonamide bound to human GluR2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-11-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.800 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 51.181, 104.548, 49.441 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3r7x |
RMSD bond length | 0.009 |
RMSD bond angle | 1.181 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.082 | 1.900 |
Rmerge | 0.062 | 0.042 | 0.141 |
Number of reflections | 21361 | ||
<I/σ(I)> | 16.8 | ||
Completeness [%] | 98.8 | 89.8 | 99.5 |
Redundancy | 10.9 | 9.8 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | HANGING DROP | 7.5 | 277 | 20% w/v 2-propanol, 10% w/v PEG4000, 100 mM HEPES, pH 7.5, HANGING DROP, temperature 277K |