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3U8D

Functionally selective inhibition of Group IIA phospholipase A2 reveals a role for vimentin in regulating arachidonic acid metabolism

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-C
Synchrotron siteAPS
Beamline14-BM-C
Temperature [K]77
Detector technologyCCD
Collection date2006-08-19
DetectorADSC QUANTUM 315
Wavelength(s)0.90020
Spacegroup nameP 31 2 1
Unit cell lengths74.422, 74.422, 93.674
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution19.820 - 1.805
R-factor0.1264
Rwork0.121
R-free0.18930
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.016
RMSD bond angle1.209
Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwarePHASER
Refinement softwarePHENIX (1.7.3_928)
Data quality characteristics
 Overall
Low resolution limit [Å]37.178
High resolution limit [Å]1.801
Number of reflections28140
Completeness [%]99.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.42930.1 M Tris-HCl pH 7.4, 10 mM CaCl2, 0.5 mM beta-octyl glucoside and 4 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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