3U6F
Mouse TREX1 D200N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-25 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.886, 56.644, 68.143 |
| Unit cell angles | 90.00, 107.97, 90.00 |
Refinement procedure
| Resolution | 33.221 - 2.300 |
| R-factor | 0.2227 |
| Rwork | 0.219 |
| R-free | 0.28850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.273 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 21073 | |
| Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 0.1 M MES, 0.075M NaCl, 12% PEG 3350, 5% 1,4-butanediol, 2mM CaCl2, pH 5.5, VAPOR DIFFUSION, temperature 298K |
