3U53
Crystal structure of human Ap4A hydrolase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BSRF BEAMLINE 3W1A |
Synchrotron site | BSRF |
Beamline | 3W1A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-06 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0000 |
Spacegroup name | P 43 |
Unit cell lengths | 72.485, 72.485, 133.491 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.705 |
R-factor | 0.21654 |
Rwork | 0.213 |
R-free | 0.28427 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kt9 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.609 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.775 |
High resolution limit [Å] | 2.705 | 2.705 |
Rmerge | 0.098 | 0.376 |
Number of reflections | 17881 | |
<I/σ(I)> | 2.5 | |
Completeness [%] | 100 | |
Redundancy | 7.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.1M Sodium citrate tribasic dihydrate, 1.2M Lithium sulfate, 0.2M Ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |