3TW8
GEF domain of DENND 1B in complex with Rab GTPase Rab35
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.588, 56.563, 175.305 |
| Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
| Resolution | 24.890 - 2.100 |
| R-factor | 0.22957 |
| Rwork | 0.227 |
| R-free | 0.26871 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.620 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 66297 | |
| Completeness [%] | 94.0 | 99.3 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 0.1 M Magnesium Formate, 19% PEG 3350, 0.1 M HEPES (pH 7.4), 4mM n-Nonyl-beta-D-glucoside, 289K, VAPOR DIFFUSION, HANGING DROP |
