3TG5
Structure of SMYD2 in complex with p53 and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-10 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.97922 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.360, 71.670, 121.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.750 - 2.300 |
R-factor | 0.24215 |
Rwork | 0.241 |
R-free | 0.26317 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tg4 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.988 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 2.320 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 20928 | |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1M Hepes (pH 7.5), 25% PEG 3350, VAPOR DIFFUSION, temperature 293K |