3SZR
Crystal structure of modified nucleotide-free human MxA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-30 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.820, 134.080, 58.100 |
| Unit cell angles | 90.00, 106.34, 90.00 |
Refinement procedure
| Resolution | 34.980 - 3.500 |
| R-factor | 0.26324 |
| Rwork | 0.262 |
| R-free | 0.29540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2AKA chain B and PDB entry 3LJB chain B |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.583 |
| Data reduction software | iMOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 4.150 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 10349 | |
| <I/σ(I)> | 9.33 | 2.25 |
| Completeness [%] | 70.6 | 29 |
| Redundancy | 3.06 | 3.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293 | 7% PEG3350, 100 mM HEPES (pH 7.6), 80 mM NaCl, 2.5% 2-methyl-2,4-pentandiol (MPD), 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
