3SZM
STRUCTURE OF HUMAN MICROCEPHALIN (MCPH1) TANDEM BRCT DOMAINS IN COMPLEX WITH A GAMMA-H2AX PHOSPHOPEPTIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-15 |
Wavelength(s) | 1.0079 |
Spacegroup name | P 1 |
Unit cell lengths | 67.810, 82.230, 104.420 |
Unit cell angles | 72.10, 89.87, 80.94 |
Refinement procedure
Resolution | 31.370 - 2.630 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.046 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | DM |
Refinement software | PHENIX ((PHENIX.REFINE: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.740 |
High resolution limit [Å] | 2.630 | 2.630 |
Rmerge | 0.064 | 0.471 |
Number of reflections | 59208 | |
<I/σ(I)> | 13.57 | 2 |
Completeness [%] | 90.2 | 97.8 |
Redundancy | 2.6 | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 20% PEG 4000, 0.1M (NH4)H2PO4, 0.1M BIS-TRIS-PROPANE , PH 4.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K |