3SYZ
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in an open binary complex with dNaM as templating nucleobase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-03 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0015 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.529, 108.529, 90.524 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.543 - 1.952 |
R-factor | 0.1623 |
Rwork | 0.160 |
R-free | 0.20130 |
Structure solution method | Difference Fourier Techniques |
Starting model (for MR) | 4ktq |
RMSD bond length | 0.006 |
RMSD bond angle | 1.077 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.070 |
High resolution limit [Å] | 1.950 | 1.950 |
<I/σ(I)> | 11.23 | 2.21 |
Completeness [%] | 99.8 | 98.6 |
Redundancy | 10.9 | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 11% PEG 8000, 0.2M MgFormate, 0.1M Tris, 20mM MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |