3SYZ
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in an open binary complex with dNaM as templating nucleobase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0015 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.529, 108.529, 90.524 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.543 - 1.952 |
| R-factor | 0.1623 |
| Rwork | 0.160 |
| R-free | 0.20130 |
| Structure solution method | Difference Fourier Techniques |
| Starting model (for MR) | 4ktq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| <I/σ(I)> | 11.23 | 2.21 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 10.9 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 11% PEG 8000, 0.2M MgFormate, 0.1M Tris, 20mM MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
