3SLA
X-ray structure of first four repeats of human beta-catenin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2010-09-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.93 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 90.790, 90.790, 364.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.710 - 2.500 |
| R-factor | 0.22115 |
| Rwork | 0.218 |
| R-free | 0.27705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.884 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.510 | 2.565 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.087 | 0.087 |
| Number of reflections | 50802 | |
| <I/σ(I)> | 11.1 | 11.1 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 5.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.98M sodium acetate trihydrate, 30% glycerol, 0.07M sodium cacodylate, 30% w/v galactose , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
