3SKU
Herpes simplex virus glycoprotein D bound to the human receptor nectin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-14 |
Detector | MAR CCD 165 mm |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 188.003, 188.003, 185.004 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 4.000 |
R-factor | 0.267 |
Rwork | 0.266 |
R-free | 0.28700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2c36 1NEU AND 3ALP |
RMSD bond length | 0.017 |
RMSD bond angle | 1.747 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.100 | 4.100 |
High resolution limit [Å] | 4.000 | 4.000 |
Number of reflections | 31947 | |
<I/σ(I)> | 8 | 1.7 |
Completeness [%] | 99.9 | 99.2 |
Redundancy | 2.6 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 293 | 1.0 M Na2HPO4/KH2PO4 pH 7.2 and 300 mM NH4SO4, VAPOR DIFFUSION, temperature 293K |