3SJH

Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-21
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	67.644, 74.977, 86.393
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.880 - 1.750
R-factor	0.1709
Rwork	0.169
R-free	0.19940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sqk
RMSD bond length	0.028
RMSD bond angle	1.946
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			1.800
High resolution limit [Å]	1.750	20.000	1.750
Rmerge	0.052	0.035	0.220
Number of reflections	44860	23	3503
<I/σ(I)>	26.62	7.95	8.98
Completeness [%]	99.7	54.8	97.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	6.5	298	22% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K