3SJH
Crystal Structure of a chimera containing the N-terminal domain (residues 8-29) of drosophila Ciboulot and the C-terminal domain (residues 18-44) of bovine Thymosin-beta4, bound to G-actin-ATP-Latrunculin A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.644, 74.977, 86.393 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.880 - 1.750 |
R-factor | 0.1709 |
Rwork | 0.169 |
R-free | 0.19940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1sqk |
RMSD bond length | 0.028 |
RMSD bond angle | 1.946 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 1.800 | ||
High resolution limit [Å] | 1.750 | 20.000 | 1.750 |
Rmerge | 0.052 | 0.035 | 0.220 |
Number of reflections | 44860 | 23 | 3503 |
<I/σ(I)> | 26.62 | 7.95 | 8.98 |
Completeness [%] | 99.7 | 54.8 | 97.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 6.5 | 298 | 22% PEG3350, 0.2M MgAcetate pH6.5, 0.45M Guanidine HCl, 1% Dioxane, hanging drop, temperature 298K |