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3SHT

Crystal structure of human MCPH1 tandem BRCT domains

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRF BEAMLINE BL17U
Synchrotron siteSSRF
BeamlineBL17U
Temperature [K]100
Detector technologyCCD
Collection date2010-10-13
DetectorMARMOSAIC 225 mm CCD
Wavelength(s)0.9792
Spacegroup nameP 1 21 1
Unit cell lengths46.469, 113.665, 74.582
Unit cell angles90.00, 91.04, 90.00
Refinement procedure
Resolution32.580 - 1.950
R-factor0.2041
Rwork0.202
R-free0.24230
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2ing
RMSD bond length0.014
RMSD bond angle1.401
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwarePHASER (2.1.4)
Refinement softwareREFMAC
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0001.980
High resolution limit [Å]1.9505.2901.950
Rmerge0.0450.0240.212
Number of reflections54633
<I/σ(I)>13.4
Completeness [%]97.197.387.5
Redundancy3.43.42.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.5281100mM HEPES, 10% PEG6000, 5% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K

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