3SHT
Crystal structure of human MCPH1 tandem BRCT domains
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-13 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.469, 113.665, 74.582 |
Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
Resolution | 32.580 - 1.950 |
R-factor | 0.2041 |
Rwork | 0.202 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ing |
RMSD bond length | 0.014 |
RMSD bond angle | 1.401 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
Rmerge | 0.045 | 0.024 | 0.212 |
Number of reflections | 54633 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 97.1 | 97.3 | 87.5 |
Redundancy | 3.4 | 3.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 281 | 100mM HEPES, 10% PEG6000, 5% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |