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3SHJ

Proteasome in complex with hydroxyurea derivative HU10

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2010-04-27
DetectorPSI PILATUS 6M
Spacegroup nameP 1 21 1
Unit cell lengths134.117, 300.565, 144.058
Unit cell angles90.00, 112.76, 90.00
Refinement procedure
Resolution49.765 - 2.800
R-factor0.238
Rwork0.238
R-free0.26600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1ryp
RMSD bond length0.007
RMSD bond angle1.300
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]49.7652.800
High resolution limit [Å]2.8002.800
Rmerge0.0890.508
Number of reflections250186
<I/σ(I)>9.42
Completeness [%]91.790.2
Redundancy2.72.4
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP729320 mM magnesium acetate, 11% PEG, 100 mM MES/NaOH, pH 7.0, crystals grow to their final size within a week, VAPOR DIFFUSION, HANGING DROP, temperature 293K

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