3SHJ
Proteasome in complex with hydroxyurea derivative HU10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-04-27 |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 134.117, 300.565, 144.058 |
Unit cell angles | 90.00, 112.76, 90.00 |
Refinement procedure
Resolution | 49.765 - 2.800 |
R-factor | 0.238 |
Rwork | 0.238 |
R-free | 0.26600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ryp |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.765 | 2.800 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.089 | 0.508 |
Number of reflections | 250186 | |
<I/σ(I)> | 9.4 | 2 |
Completeness [%] | 91.7 | 90.2 |
Redundancy | 2.7 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20 mM magnesium acetate, 11% PEG, 100 mM MES/NaOH, pH 7.0, crystals grow to their final size within a week, VAPOR DIFFUSION, HANGING DROP, temperature 293K |