3SFY
Cryptococcus neoformans protein farnesyltransferase in complex with FPT-II and ethylenediamine inhibitor 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Detector technology | CCD |
| Collection date | 2011-05-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 141.159, 141.159, 129.635 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 2.100 |
| R-factor | 0.17915 |
| Rwork | 0.178 |
| R-free | 0.20338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.279 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 73122 | |
| Completeness [%] | 100.0 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 290 | PEG4K, CAPSO pH 9.5, lithium sulfate, sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
