3SFX

Cryptococcus neoformans protein farnesyltransferase in complex with FPT-II and tipifarnib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-05-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.11
Spacegroup name	P 43 21 2
Unit cell lengths	142.176, 142.176, 130.096
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.470 - 2.000
R-factor	0.18107
Rwork	0.180
R-free	0.19905
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.264
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	85314
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	290	PEG4K, CAPSO pH 9.5, Lithium sulfate, sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K