3SDE

Crystal structure of a paraspeckle-protein heterodimer, PSPC1/NONO

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97973, 0.97963, 0.95372
Spacegroup name	C 1 2 1
Unit cell lengths	90.900, 67.180, 94.080
Unit cell angles	90.00, 99.96, 90.00

Refinement procedure

Resolution	19.360 - 1.900
R-factor	0.1855
Rwork	0.183
R-free	0.22800
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.830	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.097
Number of reflections	43472
<I/σ(I)>	16.3	2.1
Completeness [%]	98.8	97.8
Redundancy	7.5	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	28% PEG 3350, 0.5 M NaCl, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K