3S9O
The Focal Adhesion Targeting (FAT) domain of the Focal Adhesion Kinase showing N-terminal interactions in cis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97984 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.918, 223.983, 98.015 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.430 - 2.600 |
| R-factor | 0.23408 |
| Rwork | 0.233 |
| R-free | 0.28721 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k05 |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.008 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.430 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.107 | 0.487 |
| Number of reflections | 25973 | |
| <I/σ(I)> | 11 | 1.4 |
| Completeness [%] | 84.1 | 54 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 100 mM Hepes, 3.3M NaCl, 1% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
