3S3X
Structure of chicken acid-sensing ion channel 1 AT 3.0 A resolution in complex with psalmotoxin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-08-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 232.396, 109.442, 127.269 |
Unit cell angles | 90.00, 119.81, 90.00 |
Refinement procedure
Resolution | 29.915 - 2.990 |
R-factor | 0.2192 |
Rwork | 0.218 |
R-free | 0.24860 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s3w |
RMSD bond length | 0.004 |
RMSD bond angle | 0.676 |
Data reduction software | XDS ((VERSION May 10) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.030 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.108 | 0.725 |
Number of reflections | 55842 | |
<I/σ(I)> | 8.51 | 1.06 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 3.38 | 3.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 286 | 0.1M sodium acetate pH 5.5, 3 % Glycerol, 3% 1,6-hexanediol, 11 % PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 286K |