3S2K

Structural basis of Wnt signaling inhibition by Dickkopf binding to LRP5/6.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-13
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.0039
Spacegroup name	P 21 21 21
Unit cell lengths	96.043, 108.014, 173.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.820 - 2.800
R-factor	0.1967
Rwork	0.194
R-free	0.25060
RMSD bond length	0.003
RMSD bond angle	0.693
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.750	5.920	2.750
Rmerge	0.071	0.026	0.627
Number of reflections	47525
<I/σ(I)>	9.9
Completeness [%]	99.7	97.7	99.8
Redundancy	3.4	3.3	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	10-15% PEG3350, 100 mM Tris-Cl (pH 8.5), 100 mM Lithium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 295K