3S0I
Crystal Structure of D48V mutant of Human Glycolipid Transfer Protein complexed with 3-O-sulfo galactosylceramide containing nervonoyl acyl chain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-12 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.315, 47.454, 63.257 |
| Unit cell angles | 90.00, 125.47, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.500 |
| R-factor | 0.15648 |
| Rwork | 0.154 |
| R-free | 0.20599 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rzn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.294 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.061 | 0.444 |
| Number of reflections | 30330 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 273 | 20-25% PEG 3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
