3RTV
Crystal structure of the large fragment of DNA polymerase I from Thermus Aquaticus in a closed ternary complex with natural primer/template DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.296, 108.296, 90.381 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.894 - 1.900 |
| R-factor | 0.1643 |
| Rwork | 0.162 |
| R-free | 0.20170 |
| Structure solution method | Difference Fourier Techniques |
| Starting model (for MR) | 3m8s |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.7.1_743) |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.900 | 2.010 |
| High resolution limit [Å] | 1.900 | 1.890 |
| <I/σ(I)> | 17.27 | 2.33 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 9.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05M sodium cacodylate, 0.2M ammonium acetate, 0.01M magnesium acetate, 30% PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
