3RMC
Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03320 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 133.991, 123.767, 163.637 |
Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.000 |
Rwork | 0.222 |
R-free | 0.28200 |
Starting model (for MR) | 2dy4 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.307 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.085 | 0.034 | 0.695 |
Number of reflections | 107001 | ||
<I/σ(I)> | 12.1 | ||
Completeness [%] | 99.9 | 99.4 | 99.9 |
Redundancy | 4.2 | 4.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | 4.5 % PEG 20,000, 150 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP |