3RK3
Truncated SNARE complex with complexin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2009-10-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 75.861, 52.729, 128.712 |
| Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
| Resolution | 32.050 - 3.500 |
| R-factor | 0.27176 |
| Rwork | 0.270 |
| R-free | 0.31631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rk2 |
| RMSD bond length | 0.054 |
| RMSD bond angle | 1.280 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Completeness [%] | 94.8 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 13-15% polyethyleneglycol (PEG) 5000MME, 0.2 M ammonium sulfate, 0.01 M EDTA, 0.1 M Tris pH 7.5, VAPOR DIFFUSION, temperature 294K |
