3RK2
Truncated SNARE complex
Summary for 3RK2
| Entry DOI | 10.2210/pdb3rk2/pdb |
| Related | 3RK3 |
| Descriptor | Vesicle-associated membrane protein 2, Syntaxin-1A, Synaptosomal-associated protein 25, ... (6 entities in total) |
| Functional Keywords | snare proteins, membrane fusion, membrane protein-exocytosis complex, membrane protein/exocytosis |
| Biological source | Homo sapiens (human) More |
| Cellular location | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P63027 Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein (By similarity): P32851 Cytoplasm, perinuclear region (By similarity): P60880 P60880 |
| Total number of polymer chains | 8 |
| Total formula weight | 57628.70 |
| Authors | Kuemmel, D.,Reinisch, K.M. (deposition date: 2011-04-17, release date: 2011-07-27, Last modification date: 2023-09-13) |
| Primary citation | Kummel, D.,Krishnakumar, S.S.,Radoff, D.T.,Li, F.,Giraudo, C.G.,Pincet, F.,Rothman, J.E.,Reinisch, K.M. Complexin cross-links prefusion SNAREs into a zigzag array. Nat.Struct.Mol.Biol., 18:927-933, 2011 Cited by PubMed Abstract: Complexin prevents SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. To understand the mechanism for this inhibition, we determined the structure of complexin bound to a mimetic of a prefusion SNAREpin lacking the portion of the v-SNARE that zippers last to trigger fusion. The 'central helix' of complexin is anchored to one SNARE complex, while its 'accessory helix' extends away at ~45° and bridges to a second complex, occupying the vacant v-SNARE binding site to inhibit fusion. We expected the accessory helix to compete with the v-SNARE for t-SNARE binding but found instead that the interaction occurs intermolecularly. Thus, complexin organizes the SNAREs into a zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion. PubMed: 21785414DOI: 10.1038/nsmb.2101 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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