3RK3

Truncated SNARE complex with complexin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Vamp2	polymer	37	4254.8	1	UniProt (P63027) Pfam (PF00957) In PDB	Homo sapiens
2	B	Syntaxin 1a	polymer	65	7551.5	1	UniProt (P32851) Pfam (PF05739) In PDB	Rattus norvegicus
3	C	SNAP25	polymer	81	9500.6	1	UniProt (P60880) In PDB	Homo sapiens
4	D	SNAP25	polymer	65	7427.3	1	UniProt (P60880) In PDB	Homo sapiens
5	E	Complexin-1	polymer	63	7235.1	1	UniProt (O14810) Pfam (PF05835) In PDB	Homo sapiens (human)	Complexin I, CPX I, Synaphin-2

Sequence modifications

A: 28 - 60 (UniProt: P63027)

PDB	External Database	Details
Gly 24	-	expression tag
Pro 25	-	expression tag
Leu 26	-	expression tag
Gly 27	-	expression tag

B: 191 - 253 (UniProt: P32851)

PDB	External Database	Details
Gly 189	-	expression tag
Ser 190	-	expression tag

C: 7 - 82 (UniProt: P60880)

PDB	External Database	Details
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
Met 6	-	expression tag
Trp 83	-	expression tag

D: 141 - 203 (UniProt: P60880)

PDB	External Database	Details
Gly 139	-	expression tag
Ser 140	-	expression tag

E: 26 - 83 (UniProt: O14810)

PDB	External Database	Details
Gly 21	-	expression tag
Pro 22	-	expression tag
Leu 23	-	expression tag
Gly 24	-	expression tag
Ser 25	-	expression tag
Leu 27	Asp 27	engineered mutation
Phe 34	Glu 34	engineered mutation
Ala 37	Arg 37	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	35969.3
All*	Total formula weight	35969.3

*Water molecules are not included.