3R6S

Crystal structure of GlxR transcription factor from Corynebacterium glutamicum with cAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 31 2 1
Unit cell lengths	111.248, 111.248, 186.951
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	67.090 - 2.380
R-factor	0.21415
Rwork	0.210
R-free	0.28786
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i54
RMSD bond length	0.016
RMSD bond angle	1.719
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	67.090	5.050	2.530
High resolution limit [Å]	2.380	4.120	2.380
Number of reflections	53828
<I/σ(I)>		41.89	2.55
Completeness [%]	99.1	100	94.8
Redundancy	5.7	5.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293.15	0.1 M Morpheus buffer 3 (Tris; Bicine), 30% Morpheus GOL_P4K (Glycerol, PEG4K), Morpheus Alcohols Mix at 0.12 M, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K