3R6S
Crystal structure of GlxR transcription factor from Corynebacterium glutamicum with cAMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 111.248, 111.248, 186.951 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.090 - 2.380 |
R-factor | 0.21415 |
Rwork | 0.210 |
R-free | 0.28786 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3i54 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.719 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 67.090 | 5.050 | 2.530 |
High resolution limit [Å] | 2.380 | 4.120 | 2.380 |
Number of reflections | 53828 | ||
<I/σ(I)> | 41.89 | 2.55 | |
Completeness [%] | 99.1 | 100 | 94.8 |
Redundancy | 5.7 | 5.8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1 M Morpheus buffer 3 (Tris; Bicine), 30% Morpheus GOL_P4K (Glycerol, PEG4K), Morpheus Alcohols Mix at 0.12 M, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |