3R5F
Crystal structure of D-alanine-D-alnine ligase from Xanthomonas oryzae pv. oryzae with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 83.225, 83.225, 97.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.950 - 2.070 |
| R-factor | 0.21134 |
| Rwork | 0.208 |
| R-free | 0.27669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3e5n |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.098 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.070 |
| Number of reflections | 21461 |
| Completeness [%] | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 283 | 0.001ml of 6 mg/ml protein was mixed to 0.001ml of a mother liquor, containing 30%(w/v) PEG 4000, 0.1M Tris pH 8.0, 0.2M MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
