3QZV
Crystal Structure of BPTF PHD-linker-bromo in complex with histone H4K12ac peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.525, 38.513, 87.752 |
| Unit cell angles | 90.00, 111.20, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.999 |
| R-factor | 0.1863 |
| Rwork | 0.182 |
| R-free | 0.23420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f6j |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.159 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 2.050 |
| High resolution limit [Å] | 1.999 | 4.910 | 1.999 |
| Rmerge | 0.061 | 0.039 | 0.506 |
| Number of reflections | 13759 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 98.2 | 99 | 96.2 |
| Redundancy | 7.3 | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20% (W/V) PEG3000, 0.3 M NaAcetate, 0.1 M Na-Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
