3QX8
Crystal structure of MID domain from hAGO2 in complex with m7GpppG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2010-03-23 |
| Detector | RIGAKU SATURN 944+ |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.686, 46.967, 66.119 |
| Unit cell angles | 86.09, 73.33, 83.38 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27900 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3luc |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 17438 |
| Completeness [%] | 84.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M imidazole, 0.2 M NaCl, 0.46 M NaH2PO4, 1.84 M K2HPO4, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
