3QU6

Crystal structure of IRF-3 DBD free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-BM
Synchrotron site	APS
Beamline	17-BM
Temperature [K]	298
Detector technology	CCD
Collection date	2005-12-03
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9798
Spacegroup name	P 32 2 1
Unit cell lengths	64.908, 64.908, 157.621
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.384 - 2.300
R-factor	0.2157
Rwork	0.213
R-free	0.26260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2pi0
RMSD bond length	0.009
RMSD bond angle	1.192
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.384
High resolution limit [Å]	2.100
Rmerge	0.076
Number of reflections	25580
<I/σ(I)>	27.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	6% PEG 1K, 100 mM NaAc, 300 mM ZnCl2, 8% cadaverine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K