3QU6
Crystal structure of IRF-3 DBD free form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-BM |
Synchrotron site | APS |
Beamline | 17-BM |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2005-12-03 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9798 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.908, 64.908, 157.621 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.384 - 2.300 |
R-factor | 0.2157 |
Rwork | 0.213 |
R-free | 0.26260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pi0 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.192 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.384 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.076 |
Number of reflections | 25580 |
<I/σ(I)> | 27.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 6% PEG 1K, 100 mM NaAc, 300 mM ZnCl2, 8% cadaverine, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |