3QT4
Structure of digestive procathepsin L 3 of Tenebrio molitor larval midgut
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.42 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.634, 89.322, 70.076 |
| Unit cell angles | 90.00, 92.50, 90.00 |
Refinement procedure
| Resolution | 26.230 - 2.110 |
| R-factor | 0.16042 |
| Rwork | 0.158 |
| R-free | 0.20142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o6x |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.190 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmerge | 0.070 | 0.215 |
| Number of reflections | 20295 | |
| <I/σ(I)> | 25.22 | 6.94 |
| Completeness [%] | 99.3 | 94.7 |
| Redundancy | 6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 291 | 0.1 M sodium citrate, 0.2 M ammonium dihydrogen phosphate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
