3QRF
Structure of a domain-swapped FOXP3 dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.667, 131.234, 68.665 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.248 |
| R-free | 0.28280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a02 - NFAT + DNA only |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 1.021 | |
| Number of reflections | 35150 | |
| <I/σ(I)> | 4.15 | |
| Completeness [%] | 96.2 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.63 | 291 | 5 mM HEPES, pH 7.63, 2 mM dithiothreitol (DTT), 0.5 mM EDTA, and 150 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
