3QPB
Crystal Structure of Streptococcus Pyogenes Uridine Phosphorylase Reveals a Subclass of the NP-I Superfamily
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | P 1 |
Unit cell lengths | 92.021, 91.578, 170.180 |
Unit cell angles | 78.46, 82.33, 60.12 |
Refinement procedure
Resolution | 50.000 - 1.820 |
Rwork | 0.177 |
R-free | 0.20300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.820 |
Number of reflections | 404352 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 18% PEG 4000, 16% isopropanol, and 0.1 M sodium citrate pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |