3QI2

A Galpha P-loop mutation prevents transition to the activated state: G42R bound to RGS14 GoLoco

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-11-01
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.000
Spacegroup name	C 2 2 21
Unit cell lengths	70.003, 131.018, 203.307
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.705 - 2.797
R-factor	0.2001
Rwork	0.196
R-free	0.27260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2om2
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.705	2.944
High resolution limit [Å]	2.797	2.797
Number of reflections	18462
<I/σ(I)>	2	2
Completeness [%]	78.4	31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	Hanging drops were a 1:1 mixture of protein-peptide complex in buffer (10 mM Tris pH 7.5, 1 mM magnesium chloride, 5% (w/v) glycerol, 5 mM DTT) and well solution (1.7 M ammonium sulfate, 100 mM sodium acetate pH 5.0, 200 mM magnesium chloride, 10% (w/v) glycerol), VAPOR DIFFUSION, HANGING DROP, temperature 291K