3QI2
A Galpha P-loop mutation prevents transition to the activated state: G42R bound to RGS14 GoLoco
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 328 | 37531.8 | 2 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | C, D (C, D) | Regulator of G-protein signaling 14 | polymer | 36 | 4093.6 | 2 | UniProt (O43566) Pfam (PF02188) | RGS14 | |
| 3 | E, H (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) | |||
| 4 | F, I (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | G (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | J, K, L, M (A, B, C, D) | water | water | 18.0 | 37 | Chemie (HOH) |
Sequence modifications
A, B: 31 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Ser 27 | - | expression tag |
| Asn 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Arg 42 | Gly 42 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 83250.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1170.6 | |
| All* | Total formula weight | 84421.4 |
