3QI2
A Galpha P-loop mutation prevents transition to the activated state: G42R bound to RGS14 GoLoco
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Guanine nucleotide-binding protein G(i) subunit alpha-1 | polymer | 328 | 37531.8 | 2 | UniProt (P63096) Pfam (PF00503) | Homo sapiens (human) | Adenylate cyclase-inhibiting G alpha protein |
| 2 | C, D (C, D) | Regulator of G-protein signaling 14 | polymer | 36 | 4093.6 | 2 | UniProt (O43566) Pfam (PF02188) | RGS14 | |
| 3 | E, H (A, B) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 2 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 4 | F, I (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | G (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 6 | J, K, L, M (A, B, C, D) | water | water | 18.0 | 37 | Chemie (HOH) |
Sequence modifications
A, B: 31 - 354 (UniProt: P63096)
| PDB | External Database | Details |
|---|---|---|
| Ser 27 | - | expression tag |
| Asn 28 | - | expression tag |
| Ala 29 | - | expression tag |
| Gly 30 | - | expression tag |
| Arg 42 | Gly 42 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 83250.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1170.6 | |
| All* | Total formula weight | 84421.4 |
