3QF7
The Mre11:Rad50 complex forms an ATP dependent molecular clamp in DNA double-strand break repair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.149, 68.400, 71.111 |
| Unit cell angles | 98.64, 111.14, 92.03 |
Refinement procedure
| Resolution | 36.170 - 1.900 |
| R-factor | 0.1581 |
| Rwork | 0.157 |
| R-free | 0.19070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.073 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.630 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 64744 |
| Completeness [%] | 94.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 20 % PEG 2000MME, 0.2 M Trimethylamine N-oxide, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
