3QEG
Crystal structure of human N12-i2 Fab, an ADCC and neutralizing anti-HIV-1 Env antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979464 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 78.906, 78.906, 315.147 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.950 |
R-factor | 0.20483 |
Rwork | 0.203 |
R-free | 0.23612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rz8 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.794 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.335 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.094 | 0.795 |
Number of reflections | 43613 | |
<I/σ(I)> | 40 | 2.5 |
Completeness [%] | 99.0 | 99.7 |
Redundancy | 20.2 | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.01 M Ni (II) chloride hexahydrate, 0.1 M Tris HCl, pH 8.5, 20% Polyethylene glycol monomethyl ether 2,000, , VAPOR DIFFUSION, HANGING DROP, temperature 294K |