3QE0

A Galpha-i1 P-loop mutation prevents transition to the activated state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-01
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	106.637, 106.637, 455.062
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.441 - 3.000
R-factor	0.2491
Rwork	0.247
R-free	0.29170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y3a
RMSD bond length	0.002
RMSD bond angle	0.546
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.441	3.030
High resolution limit [Å]	3.000	3.000
Number of reflections	30772
<I/σ(I)>		2
Completeness [%]	96.7	85.4
Redundancy		3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	Hanging drops were a 1:1 mixture of protein-peptide complex in buffer (50 mM HEPES pH 8.0, 10 mM magnesium chloride, 10 microM GppNHp, 1 mM EDTA, 5 mM DTT) and well solution (17% (w/v) PEG MME 5K, 200 mM magnesium chloride, 100 mM HEPES pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K