3QB4
Crystal structure of a TGF-beta ligand-receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 63.810, 62.850, 124.991 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 24.000 - 2.280 |
| R-factor | 0.19983 |
| Rwork | 0.197 |
| R-free | 0.24135 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WAQ and chain C of 1REW |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.457 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.000 | 2.400 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.384 | |
| Number of reflections | 22044 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 97.8 | 97.4 |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1M Hepes pH 7.5, 0.2M NaCl 20% (w/v) PEG3350 , VAPOR DIFFUSION, HANGING DROP, temperature 294K |
