3PUA
PHF2 Jumonji-NOG-Ni(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.575, 65.767, 66.612 |
Unit cell angles | 90.00, 102.09, 90.00 |
Refinement procedure
Resolution | 32.883 - 1.890 |
R-factor | 0.1667 |
Rwork | 0.164 |
R-free | 0.21020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kv4 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.982 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.890 | 1.960 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.087 | 0.563 |
Number of reflections | 34150 | |
<I/σ(I)> | 14.2 | 2.2 |
Completeness [%] | 91.5 | 78.9 |
Redundancy | 5.1 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 18-30% polyethylene glycol 3350 and 100 mM NaCitrate, pH 5.2-6.0, VAPOR DIFFUSION |