3PU8
PHF2 Jumonji-NOG-Fe(II) complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.748, 98.126, 66.517 |
Unit cell angles | 90.00, 90.13, 90.00 |
Refinement procedure
Resolution | 33.869 - 1.943 |
R-factor | 0.1825 |
Rwork | 0.181 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kv4 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.930 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.870 | 2.020 |
High resolution limit [Å] | 1.940 | 1.940 |
Rmerge | 0.081 | |
Number of reflections | 60945 | |
<I/σ(I)> | 13.8 | 2.5 |
Completeness [%] | 98.4 | 94 |
Redundancy | 3.4 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 18-30% polyethylene glycol 3350 and 100 mM NaCitrate, pH 5.2-6.0, VAPOR DIFFUSION |