3PTA
Crystal structure of human DNMT1(646-1600) in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2009-03-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2827 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.800, 87.900, 113.900 |
| Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.600 |
| R-factor | 0.257 |
| Rwork | 0.257 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.730 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 19075 | |
| <I/σ(I)> | 16.6 | 1.8 |
| Completeness [%] | 99.0 | 94.8 |
| Redundancy | 7.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 6% PEG 20,000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
