3PT6
Crystal structure of mouse DNMT1(650-1602) in complex with DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Detector technology | CCD |
| Collection date | 2009-11-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.2827 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.192, 213.507, 86.274 |
| Unit cell angles | 90.00, 99.64, 90.00 |
Refinement procedure
| Resolution | 29.819 - 3.000 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.26590 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 58596 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 99.3 | 99 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 6% PEG20,000, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
