3PGW
Crystal structure of human U1 snRNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-08-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 358.420, 88.220, 150.900 |
| Unit cell angles | 90.00, 111.88, 90.00 |
Refinement procedure
| Resolution | 100.000 - 4.400 |
| Rwork | 0.299 |
| R-free | 0.34800 |
| Structure solution method | MR, MIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 4.600 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Number of reflections | 28079 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 8-10% PEG 4000, 0.1 M Trisodium citrate, 2 mM EDTA, 100 mM NaCl, 1-2% Anapoe X-305, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
