3PEG
Crystal structure of Neurofibromins Sec14-PH module containing a patient derived duplication (TD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-15 |
Detector | ADSC |
Wavelength(s) | 0.931 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 104.600, 104.600, 116.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.570 - 2.524 |
R-factor | 0.2254 |
Rwork | 0.223 |
R-free | 0.26430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.613 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.570 | 2.670 |
High resolution limit [Å] | 2.520 | 2.520 |
Number of reflections | 12507 | |
Completeness [%] | 94.5 | 75.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289.15 | 0.05M HEPES, 39% PEG 400, 0.2M (NH4)2SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K |