3P35
Polo-like kinase I Polo-box domain in complex with MQSpSPL phosphopeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Collection date | 2010-04-22 |
Wavelength(s) | 0.9334 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.893, 95.828, 65.964 |
Unit cell angles | 90.00, 116.34, 90.00 |
Refinement procedure
Resolution | 59.130 - 2.090 |
R-factor | 0.20922 |
Rwork | 0.206 |
R-free | 0.27315 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1UMW (chain A) |
RMSD bond length | 0.006 |
RMSD bond angle | 1.020 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 59.130 |
High resolution limit [Å] | 2.090 |
Number of reflections | 38299 |
Completeness [%] | 98.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium formate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |